Mitochondrial Activity (Cytochrome C Oxidase Activity Assay) Assay Kit

• Sensitive – detect cytochrome C oxidase activity as low as 0.35 milliunit

• Simple – Colourimetric based assay facilitates the easy measurement of mitochondria integrity and activity

Applications

• Detection of mitochondria in subcellular fractions

• Measurement of mitochondrial activity in subcellular fractions

• Measurement of mitochondria outer membrane integrity

Mitochondria Activity Assay Kit.

Absorption spectra of oxidized and reduced horse Cytochrome C. Units are absorbance in percent, normalised to the Soret band of the oxidized form.

 

For further technical specifications and details of kit components please contact our Technical Service team.

 Mitochondria Isolation Kit

Description

The Mitochondria Isolation Kit meets the demand for an effective method to isolate intact mitochondria (and mitochondrial protein) from tissues and cultured cells. The kit allows for the isolation of intact and active mitochondria in a fast, simple and cost effective process. Components in the Mitochondria Isolation kit are prepared with pure chemicals and with adherence to strict QC procedures. One kit contains enough reagents to perform 100 isolations (enriching mitochondria from 10-20g tissue or from 1x1010 - 5x1010cells).

• Easy and fast – protocol completed within an hour, no ultracentrifuge needed

• Reliable – superior-quality with reproducible mitochondrial isolations

• Efficient – generate highest possible yield of intact and enzymatically active mitochondria

Applications

• Isolating intact and enzymatically active mitochondria from tissues and cultured cells

• Isolating mitochondria proteins from tissues and cultured cells

     Mitochondria Isolation Kit Certificate of Analysis
 

 MitoLight® Mitochondrial Apoptosis Detection Kit

The MitoLight™ Apoptosis Detection Kit utilizes a lipophilic cation, termed as MitoLight™, as a mitochondrial activity marker. MitoLight™ is a mitochondrial dye that stains mitochondria in living cells in a membrane potential-dependent fashion. The monomer is in equilibrium with so-called J-aggregates, which are favored at higher dye concentration or higher mitochondrial membrane potential.
MitoLight™ partitions differently in healthy cells than in apoptotic cells. Therefore, it has been possible to use a fluorescence ratio-ing technique to study mitochondrial membrane potentials. In healthy cells, the dye accumulates and aggregates in the mitochondria, giving off a bright red fluorescence (l em = 585-590 nm). In apoptotic cells with altered mitochondrial membrane potential, the dye in its monomeric form stays in the cytoplasm, fluorescing green (l em = 527-530 nm), providing a ready discrimination between apoptotic and nonapoptotic cells. The fluorescence can be observed by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530) and PI channel for red aggregates (Ex/Em = 488/585).