Blotting membranes

Nytran® nylon membranes

Whatman Nytran nylon membranes are available in two formats. The Nytran N is a moderately charged nylon membrane and the Nytran SPC is a very high positive charged nylon membrane.
 

Nytran® Nylon

The Whatman Nytran N membrane is ideal for applications that require a lower charge. It is designed for Southern and Northern blotting as well as colony & plaque lifts and Dot-/Slot-blots. Nytran N is compatible with isotopic and non-isotopic detection methods.

Nytran N membrane allows for excellent signal-to-noise ratios. The membrane is cast uniformly on both sides of a support matrix, demonstrating excellent symmetry. This gives the membrane the ability to lie flat without curling. Nytran N membrane is a highly consistent membrane with uniform pore size and distribution. It is available in 0.2 µm and 0.45 µm pore sizes for optimal retention of oligos and larger DNA fragments.

Nytran® SuPerCharge (SPC)
 

Nytran SPC nylon membranes have a very high positive charge. Improvements in the manufacturing process result in a membrane with a higher density of nylon per unit area. The increased charge and greater nylon density provide increased binding sites for your samples.

Nytran SuPerCharge nylon membrane (1250x magnification)

Nytran SPC membranes show a very uniform pore size and pore distribution compared to typical nylon membranes. They are free of surface microvoids which are common in other membranes. These characteristics lead to greater reproducibility of results across a membrane and from blot to blot.

Nytran SPC membrane is cast uniformly on both sides of a support matrix, demonstrating excellent symmetry. This gives the membrane the ability to lie flat without curling.

With typical manufacturing techniques, increasing positive charge tends to increase background. Nytran SPC membranes are manufactured using a process that allows the combination of high positive charge with low background. Whether using radioactive or non-radioactive detection techniques, Nytran SPC consistently gives high signal with extremely low background.